It enables the 2D and 3D ultra-structure exploration of biological samples and soft matter, as well as the morphology and function of natural or artificial materials that play a crucial role in modern science and technology. The instrument was set up to operate at room temperature. Sample sections of TEM were observed using a Tecnai Spirit G2 BioTWIN (FEI Company). Imaging was performed on a Tecnai G2 Spirit BioTWIN (FEI, Hillsboro, OR). as well as analyzing saved data. 11. It enables study of low-contrast, beam-sensitive biological specimens, or other soft materials such as polymers. Tecnai G2 Spirit BioTWIN electron microscope (acceleration voltage: 120 kV). Figure 2. 名称 透射电子显微镜. Gatan Cryo-TEM Holder (model: 626). We maintain state-of-the-art equipment, provide access for researchers to microscopes and sample preparation equipment, and . FEI Tecnai G2 Spirit BioTwin. This 120 kV microscope has primary uses of: TEM of resin embedded samples; pre- and post-embedding immuno . Imaging was performed under a FEI Tecnai G2 Spirit Biotwin TEM (Thermo Fisher, USA) operating at 100 kV, with a PHURONA camera (EMSIS, Germany) and RADIUS 2.0 software (EMSIS, Germany). Instrument/Service Location: Cryo-Electron Microscopy Facility 型号 FEI Tecnai G2 Spirit BioTWIN. The following instruments are available in the Central Microscopy facility: • Transmission Electron Microscope. The hydrodynamic size distributions were characterized using dynamic light scattering (Malvern Zetasizer Nano ZS90). Sign-up Transmission Electron Microscope - Tecnai G2 Spirit BioTWIN The Tecnai G2 Spirit BioTWIN, a 20-120 kV W/LaB6 is a fully digital transmission electron microscope suited for general research in cell biology, structural biology and nanotechnology to study morphology as well as chemical composition. These labels are generated based on the related outputs. Available for loan - internal and external. The Tecnai Spirit TEM is available in two lens confi gurations: the BioTWIN and the TWIN. The samples were viewed with a Tecnai G2 Spirit BioTwin transmission electron microscope (FEI). Specifications: Equipment FEI Tecnai G2 20 TWIN electron microscope with LaB6 gun and Gatan Ultrascan 4k x 4k quad port digital camera FEI Spirit BioTWIN electron microscope with LaB6 gun and Gatan Ultrascan 4k x 4k quad port digital camera Zeiss Nvision 40 FIB SEM for 3D nanoscale high-resolution imaging (equipment maintained by EM facility and operated by Hess Lab) Zeiss Xradia 510 Versa A cryo transmission electron microscope (Tecnai G2 Spirit BioTWIN) is available to study individual molecules or macromolecular assemblies with a minimum of sample preparation artifacts. . For transmission electron microscopy (TEM) observation, anthers were fixed in 2.5% glutaraldehyde, post-fixed by 1% OsO 4, dehydrated, embedded in Epon812 resin, and ultrathin sectioned (60-70 nm thick), before examined with a Tecnai G2 spirit biotwin Transmission Electron Microscope. Its unique BioTWIN lens is designed for optimal contrast and allows the observation of unstained material. The Tecnai G 2 Spirit Bio TWIN, a 20-120 kV / LaB 6 Transmission Electron Microscope, is the high-contrast, general-purpose instrument that can perform the same tasks and analysis as the TWIN version, but is specifically suited for low contrast samples. 20-120 kV operational range; Gatan Orius 830 CCD camera; Auto-Gun and automatic tuning; Smart Tracking Position System For detection, gold nanoparticles are typically used as an electron-dense marker for . TWIN / BioTWIN. FEI TECNAI G2 Spirit BioTwin Transmission Electron Microscope (120Kv) with AMT BioSprint29 29 Megapixel camera system, for room temperature or cryo-EM JEOL 1400 Plus Transmission Electron Microscope, equipped with STEM, for room temperature or cryo-EM (Gift from Helmsley Foundation) JEOL JSM-IT500HR Scanning Electron Microscope FEI Vitrobot Mark VI Plunger LKB glass knife-makers Tousimis . The setup provides quick viewing on a large area and good quality and contrast digital imaging. Using TIA 1. The Tecnai's specifications are as follows: 2.5 Å point-to-point resolution, 1.02 Å line resolution, 1.4 Å information limit Samples can be unstained or stained. Map-based cloning. It is a high contrast, general purpose instrument that is specifically suited for low contrast samples. Complete transmission electron microscopy (TEM) service includes; automated processing of tissue, semi-thin and thin sectioning of cell and tissue samples, TEM imaging and digital image acquisition (FEI Tecnai G2 Biotwin Spirit TEM with a AMT Camera) and scanning electron microscopy (JEOL JCM-7000 Neoscope SEM). It enables to explore the native state of low-contrast, beam-sensitive biological specimens, or Tecnai performance No compromise on TEM performance TECNAI PLATFORM COMPATIBLITY Tecnai G2 Spirit BioTWIN and TWIN Tecnai G2 20 TWIN Tecnai G2 F20 TWIN Not EDX compatible Not cryo box compatible Correlated cryo experiments FEI's Tecnai with iCorr is the only solution capable of conducting correlative microscopy experiments under cryo . 1,010 (2,227) The power-supply unit measures 120 x 60 x 130 cm (47.2 x 23.6 x 51.2 in) and weighs 430 kg (948 lb). To determine drug release kinetics, the NP stock solution (1 mg ml 1) was added to the dialysis bag . Samples: Biological and clinical samples. For TEM, the particle suspension was air-dried on a 200-mesh formvar-coated copper grid (Electron Microscopy Sciences, Hartfield, PA) and imaged without further staining. Leica EM FC6 cryochamber. Genomic DNA Extraction. The Tecnai G² Spirit is designed to meet this purpose with a high level of performance. After B16F10 cells were cultured with kaempferol (0 and 15 μM) for 48 h, cell samples were prepared and then observed under a transmission electron microscope (Tecnai G2 Spirit BioTWIN). In vitro redox-triggered Pt release CP 5 NPs were transferred into a Float-A-Lyzer G2 dialysis device (MWCO 100 KDa, Spectrum) and drug release was conducted in PBS (pH 7.4) at 37 °C with stirring at 100 FEI Tecnai G2 Spirit Biotwin 120 kw transmission electron microscope. 规格. Transmission Electron Microscop, Tecnai G2. The Tecnai G2 Spirit is designed. The high internal usage of the scope makes it infeasible to use services at neighboring institutions. Together they form a unique fingerprint. Complete our contact form and w e will contact you back shortly to set up a meeting. The TIA program displays on the right-hand monitor, while Tecnai User Interface (which controls the TEM) displays on the left- hand monitor. Electron diffraction patterns were recorded at a nominal camera length of 540 mm, with a 1 s exposure time and 150 μm aperture. Zeiss LSM 880 (upright) Zeiss LSM 900 (inverted) • Super-Resolution Microscope (SMLM, SIM) Zeiss Elyra 7 . NMR Sample Preparation. The average size of the AuNPs was determined using Image J software [23]. Available for loan - internal and external. Tecnai G2 Spirit BioTwin transmission electron microscope equipped with a Gatan 4K 3 4K digital camera. The morphology and particle size distribution of AuNPs were characterized by TEM (120 kV biology transmission electron microscope, Tecnai G2 Spirit Biotwin/Tecnai G2 (Thermo Scientific, USA), and zeta potential measurements (dynamic light scattering nanoparticle size and zeta potential analyzer (Brookhaven Instruments Corporation, USA). UPLC-MS/MS analyses were conducted in research require fit and adequate microscopy techniques. to meet this purpose and level of performance. Vacuum system: Oil Diffusion (ODP) and Rotary for projection chamber; Ion (IGP) and Turbo vacuum pumps for column. FEI Tecnai G2 Spirit BioTwin The FEI Tecnai G2 Spirit BioTwin allows users to perform next-generation microscopy for life sciences applications. The mission of the Central Electron Microscopy Facility is to support researchers of the Max Planck Institutes of Biophysics and for Brain Research in their electron microscopy projects. 出产日期. 同类仪器. Targeted lipidomics Lipids were extracted using a modified version of Bligh and Dyer's method [15]. Our newest instrument, a Tecnai Spirit is an easy-to-use TEM designed to provide high-contrast, high-resolution imaging and analysis in life science applications. Also 2 final projector lenses. • Confocal Laser Scanning Microscopes. It is designed to provide high-contrast, high-resolution imaging and analysis with conventional thin-sectioned specimens, electron tomography, and cryoEM for hydrated samples Uses Tecnai G2 Spirit BioTwin; Tecnai 12 BioTwin (upgraded to G2) Both electron microscopes are equipped with a fully motorized computer controlled stage and a side mounted Veleta 2kX2k CCD camera. This high-contrast, high-resolution microscope operates up to 120 kV and achieves magnifications ranging from 2200x to 300,000x, yielding a 0.34 nm line . The scope will be integrated into the Morphology Unit and will . Gatan dry pumping station (model: 655). FEI Tecnai G2 Spirit BioTWIN This Lab6 emission, 80 kV microscope is optimized for examining biological specimen with either plastic or cryo sections, and routine negative stained samples. The NP morphology was visualized on a Tecnai G2 Spirit BioTWIN Transmission Electron Microscope (TEM, FET, USA). Electron microscopy images were acquired from thin sections under an electron microscope (Tecnai G2 Spirit BioTwin; FEI) equipped with a VELETTA CCD digital camera (Soft Imaging Systems GmbH). TF20: Tecnai G2 200kV TEM (FEI) OPERATION MANUAL for Basic TEM The FEI Tecnai TF20 is a 200kV FEG high resolution Transmission Electron Microscope (TEM), suitable for cryo single particles and semi-thick frozen cells or sections (up to 200nm) electron microscopy and electron tomography. Leica VT1000S . General Information TEM Imaging & Analysis (TIA) software is a rich environment with many functions that permit acquiring data (images, spectra, etc.) Zeiss SIGMA VP-FESEM. sEVs were observed under TEM (Tecnai G2 spirit BioTWIN). FEI Tecnai G2 Spirit Twin 120 kV Cryo-TEM - Room B29, Strathcona Anatomy & Dentistry Building Gatan Ultrascan 4000 4k x 4k CCD Camera System Model 895 FEI Tecnai 12 BioTwin 120 kV TEM - Room 0310, WH Wong Building HRMECs were seeded on the inner surface of gelatin-coated Transwell inserts (6.5-mm-diameter, 0.4-μm-pore size polycarbonate filter; Corning . 其高性能真空系统可实现Cryo-EM样品的无污染低温观察，同时配备低剂量图像 . The uniform 15N-, 13C-labeled TM domain (residues 7-39) of the ζ chain of the T cell receptor complex was expressed and purified as described . Cryo-TEM of vitrified samples (virusses, bacteria, small particles,.) The TM dimer was reconstituted in mixed detergent micelles consisting of a 5:1 molar ratio of DPC/SDS. Tecnai G2 Spirit. The phage sample in 2 × lysis buffer (20 mM EDTA and 0.5% SDS) was incubated with 50 μg/mL proteinase K at 56°C for 1 h. The phage suspension was sequentially treated with an equal volume of phenol . Together they form a unique fingerprint. Imaging was performed on a Tecnai G2 Spirit BioTWIN (FEI, Hillsboro, OR). FEI Tecnai G2 Spirit BioTwin. JEOL JEM 1200 EXII. Western blot analysis Western blotting was carried out as previously described [10]. It enables exploration of the native state of low contrast, beam sensitive biological specimens, or other . Sonja Welsch: Electron Microscopy. Tecnai BioTwin Spirit Allows users to perform next-generation microscopy for life sciences; designed to provide high-contrast, high-resolution imaging and analysis with conventional thin-sectioned specimens, electron tomography, and can be operated with a cryo holder. It enables exploration of the native state of low contrast, beam sensitive biological specimens, or other . FEI Tecnai G2 Spirit BioTWIN. UV-Vis spectra of samples were recorded on a Shimadzu UV3600 UV-Vis spectrophotometer at room temperature (RT). The setup provides quick viewing on a large area and good quality and contrast digital imaging. 20-120 kV operational range; Gatan Orius 830 CCD camera; Auto-Gun and automatic tuning; Smart Tracking Position System The microscope The microscope is a TECNAI G2 Spirit Bio TWIN (FEI Company) 120 kV with a Tungsten filament. Western blot. Contact Form. For transmission electron microscopy, there is a FEI Tecnai G2 Spirit BioTWIN that provides a 0.34 nm resolution, 120Kv and a GATAN digital imaging system with a HAADF detector for STEM imaging and an Oxford detector <135 eV for elelectron microscopyental analysis. Nanoparticle tracking analysis (NTA) Quantification and size determination of LDEVs were assessed by using a NanoSight NS500 instrument (Malvern). Tecnai G2 Spirit TWIN. FEI Tecnai 12 BioTwin TEM 40 wt% LaCo0.1Ni0.9O3 perovskite nanoparticles on Vulcan XC72R carbon support (false colored) (Courtesy Andres Molina Villarino) Physical Sciences Building Rm B-71 Sophisticated automation allows you to concentrate on . The uniform 15N-, 13C-labeled TM domain (residues 7-39) of the ζ chain of the T cell receptor complex was expressed and purified as described . Electron microscopy (EM) enables high-resolution visualization of protein distributions in biological tissues. for imaging and analysis. The Tecnai G2 Spirit BioTWIN will support a broad portfolio of basic and applied research and, equally important, the Tecnai and supporting facility will also be a cornerstone of the undergraduate curriculum in introductory and advanced cell biology teaching, as well as supporting independent undergraduate and graduate research. Leica ULTRACUT R ultramicrotome. Lab Instruments » FEI Tecnai G2 Spirit TEM STEM When mailing FedEx shipments please send to CAMCOR • University of Oregon • 1443 E. 13th Avenue • Eugene, OR 97403 • 541-346-6447 For map-based cloning of Dek33, 1173 homozygous dek33-N1299 kernels from the segregated F2 ears were used as the mapping population. It enables the user to explore the native state of low-contrast, beam-sensitive biological specimens, or other soft materials like polymers. Tecnai Spirit透射电子显微镜. Tecnai G2 Spirit BioTWIN Tecnai G2 Spirit. For cryo‐electron microscopy (Cryo‐EM) observation, approximately 3 μl apoVs at 1 μg/μl were applied to freshly glow‐discharged Quatifoil (300 mesh R1 . Leica TCS SP5 (inverted) Wellenlängen. RESULTS che-12 animals exhibit a restricted set of dye-uptake and behavioral defects: Amphid sensory neurons of C. elegans mutants defective in cilium formation and function fail to concentrate lipophilic dyes, such as Grid quality was assessed in a FEI Tecnai G2 Spirit BioTwin (FEI Company, Hillsboro, OR) operated at 120 kV, at a nominal magnification of 9300x, yielding a pixel size at the specimen level of 1.19 nm. Next generation microscopy. This Lab6 emission, 80 kV microscope is optimized for examining biological specimen with either plastic or cryo sections, and routine negative stained samples. was analyzed with a Tecnai G2 Spirit BioTWIN trans-mission electron microscope (TEM) equipped with an AMT 2k CCD camera at the Harvard University TEM core. TEM experiments were performed on Tecnai G2 Spirit BioTWIN electron microscope (acceleration voltage: 120 kV). The TM dimer was reconstituted in mixed detergent micelles consisting of a 5:1 molar ratio of DPC/SDS. microscopy (TEM, FEI Tecnai G2 Spirit BioTWIN, Hillsboro, OR) was utilized. DFT Theoretical calculations. The particles were examined with a Tecnai G2 Spirit BioTWIN 120 kV transmission electron microscope (FEI Company). The Tecnai G2 Spirit BioTWIN G2 lists at $659,492 but we have negotiated a very significant discount and have a quote to purchase the scope, camera and tomography stage at $600,000. The automatic Compustage and advanced software enable overnight data acquisitions of electron tomography data to be done. These labels are generated based on the related outputs. Fingerprint. After a further dissection to 70 nm, sections were stained with uranyl acetate (2 %) and lead citrate (2 %) for 5 min each and scanned with a JEOL 100S (Japanese Electronic Opticals Ltd, Japan) TEM or a Tecnai™ G2 Spirit BioTWIN TEM fitted with a Gatan Orius 1000 camera. The Tecnai Spirit TEM is available in two lens confi gurations: the BioTWIN and the TWIN. The hydrodynamic size distributions were characterized using DLS (Malvern Zetasizer . 所属品牌. Access to equipment for Electron Microscopy: JEOL 1400 plus, FEI Tecnai Spirit G2 BioTWIN, and a Leica Ultramicrotome. Transmission electron microscopy (TEM) measurements were made on a FEI Tecnai G2 Spirit BioTWIN transmission electron microscope operated at an accelerating voltage of 120 kV. Fingerprint. Explore the research areas in which this equipment has been used. Capabilities. Tecnai G2 Spirit Bio-TWIN Transmission Electron Microscope High contrast, high resolution for 20 -120 kV operation Accelerating Voltage 20, 40, 60 80 100 120 kV Beam source: LaB6 filament. Specifications The Tecnai G 2 Spirit TWIN, a 20-120 kV / LaB 6 TEM, is a general-purpose, high-resolution instrument. NMR Sample Preparation. temperature. It is a high contrast, general purpose instrument that is specifically suited for low contrast samples. The Tecnai G2 Spirit BioTWIN, a 20-120 kV / LaB6 Transmission Electron Microscope, is a general-purpose instrument that is specifically suited for low contrast samples. Home » Tecnai™ G² Spirit BioTWIN Tecnai™ G² Spirit BioTWIN The Tecnai G2 Spirit BioTWIN Transmission Electron Microscope is a general-purpose instrument specifically suited for low contrast samples.The microscope is equipped with an AMT 2k CCD camera. The FEI Tecnai G2 F20 X-TWIN Transmission Electron Microscope has a field-emission gun (FEG), scanning unit (STEM) with bright field/dark field detector, x-ray detector (EDS) for compositional analysis and CCD camera. Transmission electron microscopy—The images were obtained using TEM Tecnai G2 Spirit BioTWIN, at 80Kv, in the LME/IB of UNICAMP. It is equipped with a SIS Morada 11 megapixel CCD camera. The cells were harvested by RIPA and endogenous proteins were isolated from cell lysates, then the protein concentration was measured by BCA protein assay kit. Leica EM GP grid plunger. Tecnai™ G² Spirit BioTWIN The Tecnai G2 Spirit BioTWIN Transmission Electron Microscope is a general-purpose instrument specifically suited for low contrast samples.The microscope is equipped with an AMT 2k CCD camera. Results Bright-field and phase-contrast microscopy The grids were examined in a JEOL 1200EX or a Philips Tecnai G2 Spirit BioTwin TEM, and images were recorded with an AMT 2k charge-coupled device camera. The Tecnai Spirit G2 BioTWIN is a flexible 120kV microscope mainly used for screening negatively stained samples. Terms of loan/booking. The optics of the Tecnai G 2 Spirit - with the well proven and patented BioTWIN and TWIN concept - pro- vides sharp, high-contrast and high- resolution images at all magnifica- tions. For determination of immunostimulatory "danger" signals by ICD, expression of HMGB1 and CRT in EXO and MEXO were detected by western blotting. Tecnai Spirit. 2.8 The 5 lenses are objective, diffractive, intermediate, and projector. The microscope is a TECNAI G2 Spirit Bio TWIN (FEI Company) 120 kV with a Tungsten filament. See Equipment. In addition to physical properties, the chemical characterization of BPLP-MNPs and BPLP-Gd NPs was carried out using Fourier Transform Infra-Red (FTIR) spectroscopy (Nicolet-6700, Thermo Fisher Scientific, Waltham, MA). Terms of loan/booking. Available specimen holders: Standard single tilt holder The stringent demands of Life Science and Soft Matter. 产地 美国. Explore the research areas in which this equipment has been used. Transmission Electron Microscop, Tecnai G2. Cells were dissolved in 100 μL of lysis buffer for 20 min, followed by centrifugation at 12,000 rpm for 20 min at 4 °C. Three videos were recorded for each specimen . Sign-up 2.8. The solution was allowed to evaporate before the microscopy was performed. The Tecnai Spirit G2 BioTWIN is a flexible 120kV microscope mainly used for screening negatively stained samples. HRTEM images and EDS were obtained using transmission electron microscopy (FEI Tecnai G2 F20 S-Twinworking at 200 kV) equipped with X-ray energy-dispersive spectroscopy detector. The samples for TEM characterization were prepared by placing a drop of particle suspension in absolute ethanol on a carbon-coated Cu grid and being dried at room . Fischione tomography holder (model: 2020). The ABC [adenosine 5-′triphosphate (ATP)-binding cassette] transporter of mitochondria 1 (Atm1) from Saccharomyces cerevisiae is essential for the biogenesis of cytosolic iron-sulfur proteins, cellular iron regulation, and cytosolic tRNA thiolation (1-3).Similar functions have been reported for Atm1 homologs in mitochondria of, e.g., man, mice, plants, and other fungi (4-7). Bright-field images of paraffin and resin sections were taken using a Leica microscope at Shanghai University. 仪器介绍： Tecnai G2 Spirit BioTwin 透射电镜是一款简单易用的20kV~120kV透射电子显微镜 (TEM)，具备适用于生命科学应用所需的高衬度、高分辨率成像和分析能力。. The Tecnai Spirit BioTWIN TEM, specifi cally designed to maximize contrast in images of inherently low-contrast, native state, beam sensitive biological specimens, is well suited for exploring the 2D and 3D ultrastructure of cells and cell constituents. The microscope is offered in two, patented lens configurations: the BioTWIN for high contrast imaging or the TWIN for general high resolution purposes. Transmission electron microscopy (post-embedded immunogold). Applications: Classical TEM and TEM-Tomograpghy from ultrathin section of fixed samples embedded in resin. for Life Sciences. HSP70, TSG101, Alix and CD63 were verified by western blotting as the biomarkers of exosomes. microscope (Tecnai G2 Spirit BioTwin, FEI). Contact Us. Description: Transmission Electron Microscope with cryo- and tomography device. All lipidomic analyses were carried out on an Exion ultra-performance liquid chromatography (UPLC) coupled with a SCIEX QTRAP 6500 PLUS system. The morphology of spMEXO was observed under TEM (Tecnai G2 spirit Biotwin, FEI). 2.6. Electron Microscopy (TEM, FEI Tecnai G2 Spirit BioTWIN, Hillsboro, OR) was used to visualize particle morphology. Negative-stain screening; High-contrast, high-resolution imaging; Specifications. SDS PAGE and Immunoblotting CSF EV and corresponding EV-depleted CSF samples were mixed with an equal volume of radioimmunopreci-pitation assay (RIPA) lysis buffer (Triton X-100 1%, The camera has iTEM software from Olympus Soft Imaging Solutions for acquiring digital images. The Tecnai G 2 Spirit BioTWIN Transmission Electron Microscope (TEM) from FEI was installed at the Instituto Gulbenkian de Ciência in April 2016.. It is equipped with a SIS Morada 11 megapixel CCD camera. DFT computations were performed by utilizing Gaussian 09 D.01 software package. Tecnai™ G2 Spirit 光学系统经过特别设计，在 TEM 和 STEM 两种模式下均可提供亚纳米量级分辨率和高对比度。此外，Tecnai G2 Spirit 还根据不同的实验要求提供不同的配置可能性，例如，相关显微技术和各种三维及低温成像方法。 Capabilities. FEI Tecnai 12 G2 Spirit BioTWIN Overview: This Lab6 20-120 kV transmission electron microscope (TEM) is a high-contrast, general-purpose instrument, specifically suited for low contrast samples. In vitro Transwell transcytosis assay. Morada digital camera (EMSIS). 资产编号 2014009076. Leica UC6 ultramicrotome. FEI Tecnai G2 Spirit BioTWIN . The electron source in combi- nation with the excellent vacuum ensures a high brightness and a long life-time of both the tungsten and LaB 6 filaments. 厂家 美国FEI. The power-supply unit measures 120 x 60 x 130 cm (47.2 x 23.6 x 51.2 in) and weighs 430 kg (948 lb). Negative-stain screening; High-contrast, high-resolution imaging; Specifications. Courtesy of a grant from the Fondation Roland Bailly, the Tecnai Spirit BioTWIN was acquired and installed in CIME in autumn of 2011 as a TEM instrument dedicated to the high-throughput analysis of biological samples. This microscope has a high-contrast BioTWIN objective lens optimized for 2D and 3D data acquisition of biological samples. The Tecnai Spirit BioTWIN TEM, specifi cally designed to maximize contrast in images of inherently low-contrast, native state, beam sensitive biological specimens, is well suited for exploring the 2D and 3D ultrastructure of cells and cell constituents. Sign-up JEOL 1200EX The JEOL 1200EX is a conventional transmission electron microscope equipped with an AMT 2k CCD camera. FEI Tecnai 12 Spirit.
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